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Bovine pulmonary artery endothelial (BPAE) cells labeled with mouse monoclonal anti–a-tubulin antibody and detected using TSA Kit #7 with the HRP conjugate of goat anti–mouse IgG antibody and Alexa Fluor® 350 tyramide.

Immunofluorescence analysis of mesenchymal stem cells using Zymed Ms anti-Stro-1 (Cat. No. 39-8401) and Gt anti-Mouse-Alexa Fluor 488 (Cat. No. A21042) (green). Actin is stained with phalloidin-Alexa 594 (red) and nuclei are stained with DAPI (blue). Sample is mounted in ProLong Gold antifade reagent.

Western blot analysis of SARS CoV nucleoprotein-transfected HEK293 cells using Ms anti-SARS nucleoprotein (Cat. no. 37-5200).

"Western blot analysis of (A) KLF6 wt-, (B) KLF6 SV1-, and (C) KLF6 SV2-transfected cells using Ms anti-KLF6 (Cat. no. 37-8400)."

Proliferation of muntjac cells detected with Click-iT™ EdU.

Cell lysates prepared from MCF7 cells left untreated (1) or treated with IGF-1 (2-6) were resolved by SDS-PAGE transferred to PVDF. The membrane was left untreated (1-5) or treated with Lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with the rabbit anti-MDM2 [pS166] antibody (Catalog no. 44-1400G) for two hours at room temperature, following prior incubation with: no peptide (2), the non-phosphopeptide corresponding to the phosphope

Whole cell lysates of serum starved MDA-MB231 (1), MDA-MB468 (2), MCF-7 (3), T47D (4), SW480 (5) and SW620 (6) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-SAPK2d antibody (Catalog no. 441390M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detect

Whole cell lysates of serum starved HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5) PC12 (6), CMT 93 (7), Neuro 2A (8) and 3T3 (9) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-CK1e antibody (Catalog no. 441385M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antib

Whole cell lysates of serum starved MDA-MB468 cells untreated (1) or incubated with 10 ng/mL EGF for 5 minutes (2), 15 minutes (3), 30 minutes (4), 1 hour (5), 2 hours (6), 4 hours (7) and 8 hours (approximately 20,000 cells per lane) prepared with lysis buffer V19 were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-ErbB4 [pY1242] antibody (Catalog no. 44901M) at 0.5 µg/mL for 1 hour at room temperature. After

Whole cell lysates of A431 cells untreated (1) or treated for 15 minutes with TGFß (2), Bradykinin (3), pervanadate (4), anisomycin (5) or PMA (6) (approximately 20,000 cells/lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-ErbB2(Her2) [pS1113] antibody (Catalog no. 44900M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondar