Invitrogen - Molecular Probes

Data for O6146

412.30

pH >6, DMF

Abs

478

27,000

518

pH 3

Abs

492

85,000

518

pH 9

4.7

<1>	pK_a values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C.
<2>	Spectra are in aqueous buffers adjusted to >1 pH unit above and >1 pH unit below the pK_a.
<3>	The fluorescence lifetime (τ) of the Oregon Green 488 dye (O6146) in pH 9.0 buffer at 20°C is 4.1 nanoseconds. Data provided by the SPEX Fluorescence Group, Jobin Yvon Inc.