Invitrogen - Molecular Probes

Data for R363

235.17

pH >7, DMF

Abs

571

62,000

585

pH 9

see Notes

<1>	The fluorescence quantum yield of resorufin in pH 9.5 buffer is 0.74 and the fluorescence lifetime (τ) is 2.9 nanoseconds.
<2>	Absorption and fluorescence of resorufin are pH-dependent. Below the pK_a (~6.0), Abs shifts to ~480 nm and both EC and the fluorescence quantum yield are markedly lower.
<3>	Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.