Invitrogen - Molecular Probes

Data for F1300

332.31

pH >6, DMF

Abs

473

34,000

514

pH 5

Abs

490

93,000

514

pH 9

6.4

<1>	pK_a values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C.
<2>	Spectra are in aqueous buffers adjusted to >1 pH unit above and >1 pH unit below the pK_a.