Invitrogen - Molecular Probes

Data for B1151

520.45

pH >6

Abs

482

35,000

520

pH 5

Abs

503

90,000

528

pH 9

7.0

<1>	pK_a values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C.
<2>	Spectra are in aqueous buffers adjusted to >1 pH unit above and >1 pH unit below the pK_a.