Invitrogen - Molecular Probes

Data for C652

476.44

pH >6, DMF

Abs

512

11,000

563

pH 6

Abs

598

49,000

668

pH 10

7.6

<1>	pK_a values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C.
<2>	Spectra are in aqueous buffers adjusted to >1 pH unit above and >1 pH unit below the pK_a.
<3>	Data on pH-dependence of C652 spectra obtained at Molecular Probes. Additional relevant data are reported elsewhere.