Invitrogen - Molecular Probes

Data for C1270

453.45

pH >6

Abs

548

27,000

587

pH 6

Abs

576

48,000

635

pH 10

7.5

<1>	pK_a values may vary considerably depending on the temperature, ionic strength, viscosity, protein binding and other factors. Unless otherwise noted, values listed have been determined from pH-dependent fluorescence measurements at 22°C.
<2>	Spectra are in aqueous buffers adjusted to >1 pH unit above and >1 pH unit below the pK_a.
<3>	Values of pK_a for these SNAFL and SNARF indicators are as reported in published references.