Chapter 11 — Probes for Cytoskeletal Proteins
- Unlabeled and Fluorescent Actin
- Phallotoxins for F-Actin
- Properties of Phallotoxin Derivatives
- Alexa Fluor Phalloidins
- Oregon Green Phalloidins
- BODIPY Phallotoxins
- Rhodamine Phalloidin and Other Red-Fluorescent Phalloidins
- Other Labeled Phallotoxins, Including Eosin Phalloidin
- DNase I Conjugates for Staining G-Actin
- Probes and Assays for Actin Quantitation and Polymerization
- Assays for Quantitating F-Actin and G-Actin Polymerization
- Jasplakinolide — A Cell-Permeant F-Actin Probe
- Latrunculin A and Latrunculin B — Cell-Permeant Actin Antagonists
- Fluorescent Cytochalasins
- Cofilin — An F-Actin Depolymerizing Factor
- Assays for Actin-Binding Proteins
- Data Table
- Product List
- Fluorescent Tubulin
- Tubulin-Selective Paclitaxel Probes
- Paclitaxel
- TubulinTracker Green Reagent
- Fluorescent Paclitaxel Conjugates
- More Tubulin-Selective Probes
- Anti–α-Tubulin Monoclonal Antibody
- BODIPY FL Vinblastine
- Other Probes for Tubulin
- Probes for Other Cytoskeletal Proteins
- Phosphoinositides and Related Products
- Anti–Glial Fibrillary Acidic Protein (GFAP) Antibody
- Anti-Desmin Antibody
- Anti-Synapsin I Antibody
- Endostatin Protein
- Data Table
- Product List
List of Tables
Table 11.1 — Spectral characteristics of our F-actin–selective probes
Table 11.2 — Spectral characteristics of our G-actin–selective probes
List of Figures
Figure 11.1 — Ribbon diagram of the structure of uncomplexed actin in the ADP state
Figure 11.2 — Chick embryo fibroblasts. Actin from rabbit muscle, Alexa Fluor(R) 568 conjugate and coumarin phallacidin.
Figure 11.3 — Actin filaments of chick heart fibroblasts stained with rhodamine phalloidin
Figure 11.4 — Fixed, permeabilized bovine pulmonary artery endothelial cells labeled with Texas Red(R)-X phalloidin.
Figure 11.5 — Actin filaments of the turbellarian flatworm, Archimonotresis sp. Alexa Fluor(R) 488 phalloidin.
Figure 11.6 — Bovine pulmonary artery endothelial cells. Alexa Fluor(R) 633 phalloidin, DiOC{6}(3) and DAPI.
Figure 11.7 — Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green(R) 488 phalloidin and Texas Red(R) deoxyribonuclease I.
Figure 11.8 — Photostability comparison for Oregon Green 514 phalloidin and fluorescein phalloidin
Figure 11.9 — Bovine pulmonary artery endothelial cells. FluoCells(R) prepared slide #1.
Figure 11.10 — Fibroblast and neonatal cardiomyocyte. BODIPY(R) FL phallacidin and Texas Red(R) goat anti–mouse IgG (H+L) antibody.
Figure 11.11 — Mouse intestine. Alexa Fluor(R) 488 goat anti–chicken IgG conjugate, Alexa Fluor(R) 568 phalloidin and DAPI.
Figure 11.12 — Cytoskeleton of a mixed population of granule neurons and glial cells. Texas Red(R)-X phalloidin and Alexa Fluor(R) 488 goat anti–mouse IgG.
Figure 11.13 — J7473; jasplakinolide
Figure 11.14 — L12370; latrunculin A
Figure 11.15 — Polymerized microtubules. Oregon Green(R) 514 tubulin.
Figure 11.16 — P22310; paclitaxel, Oregon Green 488 conjugate (Oregon Green 488 Taxol; Flutax-2)
Figure 11.17 — Microtubules in viable HeLa cells visualized with Oregon Green(R) 488 paclitaxel.
Figure 11.18 — P7500; paclitaxel, BODIPY FL conjugate (BODIPY FL Taxol)
Figure 11.19 — Bovine pulmonary artery endothelial cells. Alexa Fluor(R) 488 phalloidin, anti–α-tubulin and Alexa Fluor(R) 594 goat anti–mouse IgG F(ab'){2}.
Figure 11.20 — Bovine pulmonary artery endothelial cell. Anti–bovine α-tubulin mouse monoclonal antibody and Alexa Fluor(R) 532 goat anti–mouse IgG.
Figure 11.21 — Zebrafish retina. Anti–bovine α-tubulin, biotin-XX conjugate; TSA Kit #22; Alexa Fluor(R) 647 goat anti–mouse IgG (H+L) conjugate; SYTOX(R) Orange nucleic acid stain.
Figure 11.22 — Bovine pulmonary artery endothelial cells stained with Alexa Fluor(R) 350 phalloidin, an anti–α-tubulin antibody and the anti–cdc6 peptide antibody.
Figure 11.23 — A prometaphase muntjac skin fibroblast stained with Alexa Fluor(R) 350 phalloidin, an anti–α-tubulin antibody and an anti–cdc6 peptide antibody.
Figure 11.24 — V12390; vinblastine, BODIPY FL conjugate (BODIPY FL vinblastine)
Figure 11.25 — Rat brain cryosections labeled with the red-fluorescent Alexa Fluor(R) 594 conjugate of anti–glial fibrillary acidic protein antibody.
Product Highlights
